https://repositorio.pucsp.br/jspui/handle/handle/26174 Wed, 29 Apr 2026 14:03:27 GMT 2026-04-29T14:03:27Z https://repositorio.pucsp.br/jspui/handle/handle/46693 Título: Desenvolvimento de scaffolds por impressão 3d de poli(L-co-D,L-ácido láctico) com citrato de trietila para potencial aplicação em prótese de menisco Abstract: The meniscus is a fibrocartilaginous structure essential for knee stability. Meniscal lesions are highly prevalent and may result from acute trauma or degenerative processes. Despite advances in therapeutic approaches, high failure rates frequently lead to chronic pain and impaired mobility. In this context, tissue engineering emerges as a promising alternative, based on the use of biomaterials capable of mimicking the natural microenvironment of the meniscus. Among the polymers employed, poly(L-co-D,L-lactic acid) (PLDLA) stands out due to its biocompatibility and biodegradability. The incorporation of the plasticizer triethyl citrate (TEC) into PLDLA is proposed as a strategy to optimize 3D printing processing and enhance cell adhesion. Associated with these properties, 3D printing has become a key technology, as it enables the fabrication of personalized implants tailored to the implantation site and individual anatomical variations. The present study aimed to develop PLDLA scaffolds containing different concentrations of TEC (1% and 2%). Computational models were designed, and the scaffolds were fabricated via 3D printing. The samples were characterized by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), zeta potential, contact angle, and compressive mechanical testing. The hydrolytic degradation of the scaffolds was evaluated through mass variation, molar mass, TGA, DSC, and scanning electron microscopy (SEM). Biological evaluation included cytocompatibility assessment using the MTT assay with the L929 cell line. The results confirmed the incorporation of TEC into PLDLA, as evidenced by FTIR, DSC, and TGA analyses, without significant changes in the thermal behavior of the polymer. A slight modification in surface charge was observed with TEC addition, making it more negative while maintaining its hydrophilic character. The incorporation of the plasticizer reduced scaffold stiffness, imparting greater flexibility to the material. However, PLDLA/TEC scaffolds exhibited mitochondrial activity after 72 hours of cell culture. Overall, the results indicate that the incorporation of TEC into PLDLA is a promising strategy in regenerative medicine, contributing to the development of PLDLA/TEC-based three-dimensional devices with potential application as meniscal prostheses Tipo: Dissertação Fri, 27 Feb 2026 00:00:00 GMT https://repositorio.pucsp.br/jspui/handle/handle/46693 2026-02-27T00:00:00Z https://repositorio.pucsp.br/jspui/handle/handle/46519 Título: Desenvolvimento de um material osteoindutor a base de amido e polímero bioabsorvível Abstract: In recent years, there has been significant progress in the field of tissue engineering and regenerative medicine, in which researchers from various disciplines seek alternatives for the development of biological substitutes aimed at replacing or regenerating diseased or damaged tissues. A promising strategy for bone tissue regeneration involves the use of scaffolds composed of bioabsorbable polymers combined with materials capable of stimulating bone regeneration. This study aims to develop a material with osteoinductive properties agent based on starch, Poly(Llactide-co-D,L-lactide) (PLDLA), and Poly(L-lactide-co-D,L-lactide-co-Trimethylene Carbonate) (PLDLA-co-TMC). To this purpose, four different formulations were prepared: I - PLDLA + 0.5% starch; II - PLDLA + 0.5% starch + PLDLA-TMC (5%); III - PLDLA + 0.5% starch + PLDLA-TMC (10%); and IV - PLDLA + 0.5% starch + PLDLATMC (15%). Dimethyl sulfoxide (DMSO) was used as the solvent, and membranes were obtained via solvent evaporation. To date, the samples have been characterized using Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). Preliminary results indicate that the addition of PLDLA-TMC to the PLDLA/starch blend alters both the thermal and morphological properties of the system, as demonstrated by TGA, DSC, and SEM analyses. However, FTIR did not show any absorption indicative of indicating chemical interaction between the components. The analysis of mass loss during the degradation process is currently ongoing and has not yielded conclusive results so far. Additionally, the samples will be characterized regarding rheological behavior, zeta potential, mechanical bending tests, atomic force microscopy, and biological assays through the response of osteoblastic cells in the presence of the materials developed Tipo: Dissertação Tue, 18 Nov 2025 00:00:00 GMT https://repositorio.pucsp.br/jspui/handle/handle/46519 2025-11-18T00:00:00Z https://repositorio.pucsp.br/jspui/handle/handle/44445 Título: Desenvolvimento de um hidrogel à base de celulose e quitosana contendo Óleo Essencial de Melaleuca no tratamento de onicomicose causada por dermatófito e levedura Abstract: Onychomycosis is an infection caused by dermatophytic fungi and yeasts that affects 20% of the global population and accounts for 50% of all nail diseases. Currently, alternative therapies under investigation for the treatment of onychomycosis include the use of essential oils as potential antifungal agents. Objective: To develop a film[1]forming hydrogel combining hydroxypropyl methylcellulose (HPMC) and chitosan, associated with Tea Tree Essential Oil (TTO) at concentrations of 1%, 2%, and 5%, for the treatment of onychomycosis. Methodology: For material characterization, the following analyses were performed: Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TG), and Differential Scanning Calorimetry (DSC). For microbiological analyses, sensitivity testing was conducted by determining the inhibition zone and comparing it to standard antifungal agents (antifungigram), along with microbial growth inhibition analysis through optical density measurement, which assesses microbial growth in liquid medium by turbidity reading. Results: FTIR analysis showed no chemical interaction between the components, which is important to ensure the release of TTO for therapeutic purposes. DSC analysis indicated that increasing the TTO concentration reduced the interaction between TTO and the hydrogel components (HPMC and chitosan), facilitating the evaporation of TTO. TG analysis revealed that TTO concentration did not affect the thermal properties of the samples. In the microbiological analysis, the 2% and 5% TTO concentrations exhibited antifungal activity, which was more pronounced against yeasts. Conclusion: Considering these findings, compounded formulations based on cellulose and chitosan with Tea Tree Essential Oil for nail film formation are potential tools in the treatment of onychomycosis caused by dermatophytes or yeasts. In addition to being a natural, low[1]cost, and low-toxicity therapeutic alternative, future clinical trials are crucially important to confirm safety and efficacy Tipo: Dissertação Thu, 22 May 2025 00:00:00 GMT https://repositorio.pucsp.br/jspui/handle/handle/44445 2025-05-22T00:00:00Z https://repositorio.pucsp.br/jspui/handle/handle/44442 Título: Avaliação da regeneração óssea craniana utilizando scaffolds baseados em Poli(L-co-D, L-ácido lático-co-trimetileno carbonato): estudo in vitro Abstract: Craniofacial bone regeneration represents a significant challenge in regenerative medicine due to the anatomical and functional complexity involved in repairing defects resulting from trauma, surgery, or congenital malformations. In this context, the development of bioabsorbable polymeric scaffolds has emerged as a promising strategy to promote effective and personalized bone regeneration. This study aimed to synthesize and evaluate in vitro three-dimensional scaffolds based on poly(L-co-D,L-lactic acid-co-trimethylene carbonate) (PLDLA-TMC), with potential application in cranial bone regeneration. The copolymer was synthesized and processed using sucrose as a porogenic agent in two pore size ranges (125~250 µm and 250~500 µm), followed by 3D extrusion printing at 150 °C in two thicknesses (0.8 and 1.2 mm). After printing, the scaffolds were immersed in distilled water to remove the sucrose and generate a porous structure. FTIR analysis confirmed successful copolymer synthesis, and rheological tests indicated pseudoplastic behavior. Thermogravimetric analysis (TGA) of the scaffolds revealed a shift in the onset temperature of mass loss after the printing and leaching processes. However, the DSC analysis did not show significant changes in the glass transition temperature (Tg) for the printed scaffolds. Zeta potential analysis demonstrated a pH-dependent negative surface charge. Scanning electron microscopy (SEM) revealed morphological differences related to thickness and pore size. Based on these analyses, scaffolds printed with thicknesses of 0.8 and 1.2 mm and pore sizes between 125~250 µm were selected for in vitro biological assays. The bioluminescence assay showed higher luciferase signal expression in the scaffolds with 1.2 mm thickness. Confocal laser scanning microscopy and SEM demonstrated good cell adhesion and proliferation on both materials, with osteopontin expression throughout the scaffold structure. Therefore, the set of results indicates that the printed PLDLA-TMC scaffolds exhibit favorable physicochemical and biological properties, making them promising candidates for craniofacial bone tissue engineering applications Tipo: Dissertação Wed, 14 May 2025 00:00:00 GMT https://repositorio.pucsp.br/jspui/handle/handle/44442 2025-05-14T00:00:00Z